#plotonic of course

[opt1mistic]

HIGH AND DRY

ellie x reader. warnings: angst, unrequited love, smoking(r), and also lowek hates herself🤷‍♀️, that’s pretty much it. also not proofread. wc: 0.5k

note: this was originally supposed to be waaaay longer but i got a wee bit scared so i decided to keep it short🙂(and i also didn’t want to write anymore)

there’s something about tonight that makes you want to spill your gut. the way the tiny stars shined from your appartement balcony. the chilly breeze flowing into your face, making your hand wrap around yourself and hold your elbows tight. the smell of the cigarette in between your two fingers was comforting, it brought peace to you. every time you bring the end of the cigarette to your slightly dry lips, the way the smoke filled up your lungs made you feel numb to the thought of losing her.

why were feeling so fucking complicated?

god, the was she look at you, her laugh, her face, everything about her was perfect. she was perfect. and you were sure she thought the same for you, but you just didn’t think you were perfect enough for her. you were always there for her though every break up, through her high and lows. but all you did was push her away when you needs a shoulder to cry on. locking all your feelings away so she couldn’t come and help you. all this pain just because you did want her to leave. you knew if you let her get close to you your feelings would grow bigger and stronger, and you knew it would end up breaking your heart.

but the one time you did need her, of course she was there. oh, how much you hated her for it. the words of “i have feeling for you” leaving your lips. her face staring blank at you. what have you done? you could tell that she didn’t like you back. the way her face morphed into a look of pitty. ellie didn’t what to hurt your feelings by saying no, but she had no other choice.

i mean don’t get her wrong she thought you were really pretty. you were the most amazing person ever. the light of her life. but all she saw you as was a sister. you were her platonic soulmate, in every universe. but was there a universe where you are more then that? plotonic?

you hated yourself for so long for ruining your friendship. seconds before you said thoes words you hoped that she wouldn’t leave you, she was the best thing you ever had, (even if there was nothing romantic about it… to her at least), she was the best thing that ever happened to you. and you ruined it—with something as ‘silly’ as love.

your lips parted, letting up the smoke from your lungs. you shut your eyes are you let all the smoke out, and when you opened your eyes, a few salty tears fall down your cold cheeks, scattering at the top of your chin. each one of those tears carried unspoken words you couldn’t say, everything you’ve been holding back.

snorting back some snot that was threatening to slip from your nose, you reach your free hand to wipe your face free of tears. you put out the cigarette and walked back into your apartment, the scent of smoke and salt still clinging to you, as this night refused to let you ever forget.

[lbcreations-blog]

Hello I want to request an imagine or Request a scenario or your opinion on it!

If lucifer and Liliths had a child that was an angel( not fallen, maybe same powers as lucifer. Edits he fell). Full angel looking with no demon blood. What would lucifer do? What would the angels or god do if they discovered this? Would they take the child to be raised in heaven or let it be? What if she had the divine power unlike her father to transport herself between those worlds and ended up in heaven accidentally and meet angels or god. ( she was sheltered and didn’t know about heaven and the extermination). I will not leave further questions but see what you make of it.

Have a good day!

You gave me such a big request but I'll try my best

I also decided to make this a two part thing because I felt like it. I hope you don't mind

Note: Since you weren't specific if you wanted this a reader type story or not, I made this a reader story since I'm used to that. I also apologize for this coming out so late, but yoh, this was long

Characters' relashionship with the reader is: plotonic

Possible spelling mistakes

Gn reader

Masterlist

To pure for damenation

Your reading part 1 | part 2 |

You were the youngest of the morningstars. By some miracle yet also curse, you were born with all of your fathers angelic features, you had his wings six of them like him, you had his what some would call angelic aura, and all of his angelic powers, shokenly you had a halo as well, which Lucifer did not have the heart to get rid of and for your body to feel such pain.

You were basically a demonic Seraphim if you want to put it that way, even though the only demonic feature you had was the sharp teeth that your father had. Any other feature you had was your mother's, for instance, your purple eyes.

When both of your parents realized you had about 1% demonic features and the same going from your soul, they both knew heaven might want to snatch you up if they knew or you would want to go to heaven if you knew what it was.

Now they both knew that they had to shelter you from heaven and the extermination, and possibly charlie aswell until she's old enough to keep it a secret from you, which was not far away in this timeline, she is about 15 years older then you in human years.

We now skip ahead into the future, where now you are about 10 years old, your mother's gone, Charlie has apparently gone off to do some dream of hers that you are not aloud to know and your dad has also bloody joined that masquerade. He goes to work there and then comes back to you when that's done.

But he hardly hangs out with you anymore. Of course, it was hardly a difference to when he had seen Charlie yet. But he actually let you watch him work when you got bored. But now you're not even aloud to do that because, for some reason, you're too young to know what Charlie's working on except the fact that it's a hotel.

I mean, you know what sexual reproduction is. You do neutral science, and you have seen a few horror movies, so you know what gore is, so what is going on? You needed to know. So you were going to find out. When your father goes to bed at night, you'll sneak out and find that hotel. So that's what you did.

Once you got out of the palace, you were terrified of the creatures you saw. This was only the first time you were out of the palace walls and saw the city. The only other times you were out of the palace, you were just telaported to the restaurant and such.

While walking to where you thought the hotel was, you tried asking a sinner where the hotel was, but they tried hurting you, so you ran in the original direction you were going. You ran so fast that you almost ran into the statue in front of the hotel.

You looked up at the hotel. The hotel was huge, you looked to the left of it and saw an apple, you knew that was your dad's office. But before you could get into the hotel, you saw a weird looking flying gold thing. You watched as it landed in front of what you assumed was a weird looking man sleeping in gold liquid.

You saw someone with wings, a weird thing you assumed was a mask and a circle above their head, walk out and pick up the man, until suddenly they looked at you. Through the mask, they seemed shocked, and you weren't sure why.

They turned to the side, and from what you saw, they were talking to someone else in the ship. Then, someone who looked like the one who was holding the man came out. They suddenly walked towards you. "Hi!" You said to the stranger, thinking they wanted to have a friendly talk with you.

Instead, they grabbed your arm and told you to come with them. "Huh? Why would I do that?" You asked, wanting to know why you should follow a stranger. They said something on how they didn't know how you got out and an apparent woman being worried about you.

"Oh, do you mean my mother?" You asked, thinking they meant Lilith your mother. They said yes as they pulled you towards the ship. And suddenly, you guys were up in the air and went into a portal.

That's the end of part one. Who are these mysterious strangers that took you from your home? Where did the portal take you? And will you come back home? Find out next in To Pure For Damention part 2

Taglist: @fatherlesschild2 @whitewingsh @iheartpieck @i-yuki

Taglist request section

-L.B Creations

[myafterlifeisbetterthenyours]

A part 2 to either of the girlfriend abuses you series

where we se the recovery or the family of the girlfriend comes after them

People we love

Octava stared down at you, the girl in silent contemplation as you snuggled into her chest, the young lady holding you close.

She ran a hand through your hair, happy to see It'd been growing back well in the small time you'd been staying with her and her family.

As per her mother's Insistence you'd been sharing not just a room, but also a bed for the past few weeks and you'd noticeably improved.

You'd become happier. Eyes brighter. More and more welcoming every day, started smiling again.

Not those forced, people pleasing smiles she now recognised from before.

You weren't as touchy feely as before, but you weren't afraid to sit next to her, or hug her anymore.

Hugs.

You started hugging her again.

She was surprised the first time. You just randomly hugging the girl one morning, holding her for several minutes before youd part, thanking her softly as you walked off.

Since then, it'd been a pleasant surprise throught the past few weeks.

She'd missed the contact with you, desperately so.

Youd always been affectionate with her and just being able to hold you close again was a treat. Especially with you being the one to initiate it. It meant the world to her.

You'd also been sharing her bed, as per her mother's arrangement.

And while she'd initially been flustered and slightly uncomfortable, she'd quickly gotten used to it, the girl coming to enjoy it.

Plotonically, of course. You had slept over many times before.

Well, before you began dating HER!

But you were free now.

Free from her and her horrible and nasty ways. And she'd be dead and buried before she ever let that BITCH lay so much as a finger on you again.

You were stand-offish in bed, then again, you were stand-offish most of the day, which made sense, as you were still recovering.

But it'd be the second week you shared a bed that things... shifted.

You'd kept your distance the first few days, sleeping on the other side of the bed, telling her goodnight softly before curling in on yourself, sleeping tight, keeping clear away from her.

As you'd done the last several days, you all curled up on the other side of the bed, she'd started longing for your warmth.

Or at least that's what she'd thought, as it'd been the fifth day before you'd head to bed, the two of you getting into bed, you saying goodnight as usual.

Octavia saying it in turn, rolling over to sleep.

But itd be after several minutes of silence that she felt you shift, shuffling across the mattress, Octavia rolling over as you practically latching onto her, pulling her close in an iron grip, hugging her stomach as you laid there.

Octavia laid there for a minute, breathing deep before a smile crossed her face, tears pricking her eyes as she pulled you close, the two of you laid there, Octavia so happy to have you bridge the gap.

That became the norm for the two of you.

You never addressed the situation, never talking about it, it becoming something of a nightly ritual.

You'd say goodnight, the two of you laying there for several moments before you shift and roll, quickly shuffling over to her, holding onto her.

Octavia loved it, the young lady already turned towards you, a smile on her face, just waiting for you to approach.

And like clockwork you turned over, shuffling until you were pressed against her, hugging her core, Octavia smiling softly as you held her close, nuzzling her fluffy chest.

You'd hold onto her with a terrified grip, and while she normally hated overbearing grips, she could tell you needed it, practically relishing the way you clung to her.

Loving it as she held you close, softly stroking your hair as you just hugged her, the young lady knowing it couldn't be easy recovering from what you'd been through.

And it was a month in where the scene began.

Octavia waking up before you, the young lady finding you in her grasp, relishing the contact you shared, the early morning sun seeping into her room as she held you close, the young lady softly stroking your growing hair, relishing the warmth and comfort you granted her.

She breathed softly, the owlet watching your sleeping face, watching as your expression tensed, breathing picking up as you stirred, shuffling, hands gripping her tight, nails digging into her back as you held her tight. Almost painfully so.

Octavia went ridged as you jerked, letting out a strangled.

"...No!"

Your arms gripping holding her like a vice.

"Please... no!"

Your voice was pained... desperate. You clinging to her as if she were the last being in Hell.

You'd whine and whimper for another minute before awakeinging.

You'd jerk awake, releasing a desperate, "NO!" Snapping up as you grabbed at the sheets.

Tears streamed down your face as you sat there, breathing heavily as Octavia sat there stunned, watching you, the young lady sitting up as she carefully reached out to you.

Placing her hand on your shoulder you snapped to her, spinning around and almost falling out of her bed.

You had tears in your eyes as you stared at her, expression horrified as you sat there.

The girl barely processed what had happened when you launched at her, grabbing the girl as you buried your face in her chest.

"I'm sorry! I'm so sorry..." You sobbed, clinging to her, tears wetting her chest. "Please, don't leave me..."

You sobbed, whining pathetically, clinging to her desperately, face buried in the nook of her neck as you sobbed, apologising over and over as you sobbed loudly.

Octavia, snapping out of her shock, quickly held you close, shushing you over and over as you sobbed, the girl unable to even imagine what you'd been through, the young ladies attention solely on you as she did her absolute best to comfort you.

Holding you close, stroking your back as she hugged you, kissing the top of your head, telling you it was alright, over and over again.

You'd stay like that most the morning, you apologising over and over, clinging to her desperately as the two of you just laid there, Octavia doing her best to comfort you as you quickly soaked through her shirt.

It was almost lunchtime by the time she'd finished comforting you, Octavia fiddling her shirt as she sat in front of her, well, 'meal".

The soaked material on her chest holding a far greater weight then simply tears.

You'd all but passed out after your breakdown.

You'd clung to her for what felt like hours, you apologising over and over, sobbing like a baby as you clung to her.

Octavia unable to do anything but hold you close, assuring you over and over you weren't alone. That you didn't need to apologise. That she was there for you.

Not that it seemed to calm you.

You sobbing and crying for seemingly hours before all bit collapsing.

She'd left you to sleep.

She wished to stay with you, she truly did, but she was hungry and needed a moment to herself, the young lady knowing her just sitting around you wouldn't do any good.

So she'd gone to eat, the young lady sat before her meal, mind a million miles away as she played with her meal.

"Ah, hello dear, how are you this morning?"

Stolas' elegant Goetian voice cut the silence, Octavia perking up as she looked up in time to see the man waltz in.

Octavia just sat there sternly, tired and emotionally stressed, playing with her food before she looked up at the man.

"What's going to happen?" She asked softly, an edge to her voice.

Stolas paused, head turning towards the young lady, the demon Prince staring for several moments before piping up.

"Well, uhhh, I was planning on a late breakfast, then-"

He tried to play it off casually only for Octavia to cut him off, coarsely asking him.

"The Bitch!" She snapped, rising to her feet. "What's going to happen to the fucking bitch!"

She seethed, breathing heavy, Stolas taken aback by his daughters outburst.

"Via, darling, me and your mother are handling the situation, trust u-" he tried to deescelate the situation, only from Octavia to snap at her father.

"No! No!" She growled, shoving her meal away. "Look, I haven't brought it up cause I was sure you and mum had something planed, but you haven't seen what I've seen, okay? (Y/N) has been through enough! I need to know he's safe!"

She breathed heavily, gripping their kitchen table.

"(Y/N) deserves more... He deserves... He deserves safety... and we can't give him that until she's dealt with."

She finished, breathing heavy, staring down at the table as memories of your breakdowns flashed through her mind, the young avian gripping her still soaked shirt, emotions spiking violently.

Stolas paused for several moments before walking over, the man hugging the young lady, the owl prince cooing softly.

"I know this has been a lot darling, but I promise, It's all going to be alright. Don't worry... we'll deal with her soon. Everything will be okay. So trust me, were taking care of it my dear little starfire."

Octavia stood there for several moments before sighing, nuzzling her fathers chest, sighing as she leaned against the mans fuzzy chest, shoulders slumping as she released the steadily building tension, the young lady having so much on her shoulder since you'd been placed in their charge.

The two stood for several moments, hugging each other close before your voice suddenly cut in.

"Uuum, I'm not interrupting am I?"

At that the pair turned, finding you stood in the kitchen doorway, hugging yourself, in a rather pathetic state, Stolas stepping back as Octavia perked right up.

You looked exhausted.

Bags under your eyes, growing hair in a mess of 'bed-head', clothes dishevelled as you looked at the pair.

"N-no, not at all my boy, please come in!" Stolas stumbled, quickly inviting you into the room, doing his best to raise the rooms spirits.

You'd do so, walking in, still holding yourself as you got yourself a drink of juice, taking a seat besides Octavia.

The girl was tense, though quickly relaxed as you shared a smile with the girl, a soft, tender smile on your face as you sipped your drink.

Octavia quickly returned the gesture, the tension in the air quickly disappearing as the two of you sat there.

Stolas would chat up the two of you, you speaking sparingly, clearly not in a speaking mood, Octavia's gaze rarely leaving you as she discussed the days plan with her father.

The three of you would finish your respective 'meals'. You once again washing your mug, Octavia sticking close this time, an ache filling her gut as she noticed the fading bruises on your forearms.

She was happy to see them fading, knowing you were healing was a major comfort to her, but even then, it still hurt knowing you'd suffered as you did.

The girl holding herself responsible on some level.

Finishing up in the kitchen the three of you'd end up in the 'greenhouse', you sat amongst several of Stolas' plants.

You and Stolas happily discussed said plants, you genuinely smiling as one of the larger plants nuzzled up to you, you practically beaming as you discussed the various aspects of her fathers unique plant collection.

Octavia just sat on a nearby arm chair, smiling softly as you seemed to come out of your shell, right at home as you discussed the man's carnivorous flora.

The young lady smiling as she watched you engage with her father, practically beaming as saw your eager, giddy nature seep out, reminding her oh so much of what you were like before...

Before you met that Bitch!!!

You'd be discussing the unique features of a carnivorous plants when youd be interupted by an imp servant, the servant whispering something into the mans ear before Stolas would stand up staight.

Clearing his throat, the man would excuse himself, telling you something urgent had come up and he'd be right back.

And so, youd be left alone, you playing with one of his plants before shed turn to you.

She was about to ask you about your favourite part of fathers collection when a series of violent slamming sounds rang out.

The two of you froze, Octavia on her feet just as the shrill voice streeched out.

"Where the fuck is my Boyfriend!"

An all too familiar voice screeched, the girls feathers standing on alert, Octavias heart beating like a war drum as you instinctively pulled in on yourself, the plant on your lap pulling itself away.

You froze, eyes widening in horror, Octavia recognising that expression.

That look in your eye.

A look of utter horror and fear.

The sound of the palace doors slamming open snapped her from her state, the girl rushing over to you, stopping before you just as the heavy footsteps rushed onto the room.

You began breathing heavily, seemingly bordering a panic attack, Octavia about to reach out and comfort you when the door to the greenhouse suddenly burst open, the girl spinning around.

There she stood.

Your Ex girlfriend wasn't an ugly girl, not by any means.

At least not physically.

By all conventional standards she was a very attractive young lady.

Though Octavia couldn't give less of a shit what conventions may think.

She could have been the Queen of Hell for all she cared.

The bitch was your abuser.

And as far as she was concerned that made her the most repugnant bitch in Hell.

And if she had it her way, she'd be a dead bitch.

The young lady breathed heavily, clearly unhappy, eyes pearcing as she glared right at you, an ominous aura filling the room.

Octavia would take a defensive stance, the young lady stepping before you as you sat there holding yourself, frozen, shaking like a leaf as the young lady put herself in between you and your abuser.

"Come here. Now." The young lady ordered, her voice suspiciously level, eyes glaring, almost as if she were putting on an act.

Looking back at you, your eyes were glued to the floor as you gripped yourself, all but hiding behind Octavia, unable to gather the will, or courage to do anything but sit there.

"Darling." The girl began, voice suspiciously sweet. "I haven't seen you in weeks, I just wish to make sure your alright."

She spoke in a sinisterly sweet tone, extending her arms for a hug.

The girl suddenly all calm and seeet as if she were some caring figure. As if she weren't the bitch who abused you for Lucifer knows how long.

"He doesnt-" Octavia went to speak for you, wanting to tell her off, only for the other young lady to snap at her, telling her curtly.

"I wasn't talking to you!" She spoke, contempt thick in her voice. "I was talking to my darling boyfriend~"

She cooed, smiling to you, a sinfully sweet smile crossing her face as she softly held her hands out.

Octavia just sneered, planting her feet, intent on telling her off, but before she could speak you stepped forwards.

Octavia stared in horror, breath caught in her chest as she stared, the girl turning, raising an arm, about to say something when you looked at her, smiling softly before stepping before the girl.

The young lady just smiled, a sisnister glint to her eye that made her skin crawl, the girl smiling at you as you stood before her. Youd take a deep breath, composing yourself as you stepped to her.

But after a moment, you took a deep breath, and looking back at the girl with a surge of integrity, you turned to your 'girlfriend', telling her curtly.

"I think you should leave."

Your voice shook, fists clenching the hem of your shirt as you did your best to look at the girl.

Octavia was shocked, relieved and proud to hear you stand up for yourself, but a deep sense of fear would hit her, the young lady getting an ominous feeling in her guy.

The young lady on the other hand just froze, staring at you for several moments before speaking coldy.

"What did you just say?"

She was incredulous, a sneer crossing her face as she stared down at you.

"I think..." You began, breathing heavily, heart racing as you clenched your fists, trying to stay composed. "I think you should leave... We... I think we're done!"

If the young lady had been unhappy before, she was utterly enraged now.

Before either of you could even react, the lady had slapped you, the young woman grabbing you by the hair, violently yanking at it as she knocked you to your knees.

"You dont get to think! Is that how I trained you!?" She snarled, her voice suddenly booming as she violently yanked on your hair.

You were almost immediately on your knees in tears as she tore at a clump of hair, you desperately gripping her wrist as she yanked at your scalp.

"I put all my time into making you worth something and you think you can just walk away?! You dont get to think! You useless excuse of a Goetia!"

She snarled, voice filled with such venom.

You were in tears, sobbing violently, profusely begging for forgiveness as she brutally yanked on your hair, something she had done countless times before, the reason you'd started wearing it short.

But with your hair grown out, she once again had a solid grip, the pain intense, your body seizing with pain.

Before the bitch could say another word however, Octavia stormed over, slapping the bitch right across the face.

Hard.

The bitch stumbled back, releasing you, allowing you to fall to the floor, sodding as Octavia came to your aid, pulling you close, holding you to her chest as she shielded you, you quickly latching onto the girl, sobbing violently into her chest.

Seeing you cling to the girl, apologising profusely into her cheat, the bitch would become enraged.

Storming over to the girl as she held you, the bitch would raise her hand, intent on slapping Octavia, fire in her eyes as she stood over the young goetia.

However before she could strike the girl, her hand was suddenly grabbed, the bitch looking up to find none other then Stolas of the Ars Goetia standing over her, a powerful sneer crossing his face.

His eyes glowing a sinister red.

The bitches face instantly fell, the girl stumbling back, not getting far as his grip remeaind firm as steel.

The bitch tried in vein to explain herself as Stolas only tightened his grip on her forearm, the man glaring down at her before he snapped at her.

"Shut Up!"

He'd tell her bluntly.

"You see that boy?" The man spoke, a deathly cold tone to his voice. "He doesn't belong to you."

"He belongs to me now. To my family. He belongs to me and my daughter. Not you..."

Octavia would try and make sense of his statement, when Stolas suddenly smacked her with the revelation.

"That boy is now engaged to my daughter and I won't have anybody, least of all some spoiled little bitch daughter of a Duke abusing him."

Hearing this, the young woman's face went pale, Octavia's own eyes bulged, the revelation being a metaphorical slap to the face, even more so for her then the bitch before her.

"B-Bu-I-I, No I didn-nt."

The bitch tried to speak again, trying to justify herself to a prince of Hell, but Stolas didn't care, the man harshly twisting her arm, a sickening snap ringing out as the girl screamed bloody murder, her forearm bending at a disturbing angle.

You meanwhile just clung to Octavia, face buried in her chest, holding onto her even tighter as the bitch screeched in pain, Octavia holding you even tighter, doing her best to comfort you as you clung yo her, mind still processing her father's revelation as you flinched at the girls screeches.

The Demon Prince would drag the girl out of his Palace by her collar, the girl screeching and crying in pain all throughout.

The Bitch begged for mercy as Stolas rather ruthlessly tossed her to the street, using her amr as leverage as he threw her, the girl screeching in pain as she held into her shattered forearm, the girls yowls of pain being heard for blocks.

You meanwhile just sat in her arms, sobbing into Octavias chest, Octavia doing her very best to comfort you.

The girl both overwhelmed but also deeply concerned, the owlet pushing aside her own feelings and conflicting feelings as she did her best to help you, focusing on your emotional distress.

You'd cling to her, your violent sobs finally settling down as Stolas re-entered the room.

The man standing over the two of you for several moments before eoctavua looked to him.

"When were you going to tell me?" She'd ask softly after several minutes, not quite accusatory, but with a notable edge to her voice, her attention on you.

The man stood in silence for several moments before sighing, the avian looking down at the both of us.

"It was recent. Very recent. The finalisation only just being finished. Your mother managing to secure the agreement with his family only this morning. We were waiting for the right moment to mention it and well..."

They'd both look down at you as you desperately clung to the girl.

"He needs you dear. Now more then ever."

He'd stand there for several long moments before he'd step out of the room, the two of you being left alone.

You'd cry softly into her chest, the girl just holding you close as she gently hushed you, gently kissing the top of your head.

"... I'm sorry...." you spoke softly, gripping her arm.

Octavia would hush you, rubbing your back.

"Hey, it's not your fault." She tried assuring but you just pushed her away, wiping your eyes.

"No!" You began, barely holding back sobs. "You don't deserve this!"

You sobbed, pulling away as she reached out to you.

"You deserve a man who will protect you!" You turned, staring at her, an anger in your eyes as tears streamed down your face.

"You deserve somebody strong. And brave. And capable. And-And-Not somebody... Not somebody weak and helpless... you deserve somebody... some... thing... More..."

"And I'm... I'm just nothing..."

You finished so softly. So weakly.

The girl utterly decimated at the idea of you genuinely believing you were nothing... it was too much.

The girl would grab you, yanking you towards her as she forced you to look at her.

"Don't you dare!..." she'd sniffle, eyes welling up with tears. "You are something! You so much!"

She'd gasped, her breath shaky as she struggled keeping her composure.

"You are the best thing that ever happened to me (Y/N)! My best friend..."

She'd begin crying, furiously wiping tears from her eyes.

"Your not nothing. Your everything to me (Y/N)... You deserve so much more and I'm so, so sorry!" She'd cry, looking to him, lip quivering, breathing shaking as she looks down at you. "I should have been there! I should have protected you!"

She'd sob herself, leaning into the crook of your neck.

"I knew it... I knew she was rotten and I... I didn't stop it when I could have..."

She'd cling to you, nuzzling your neck.

"I'm so sorry (Y/N)!"

You'd just hold you, utterly bewildered.

"N-No, Octavia, you-You saved me!"

You'd pull back, looking at her.

"Y-Your the best part of me! The best thing-person I could ever ask for!"

You'd tell her, struggling to stay coherent as your breathing hitched over and over again.

"You-You deserve more, so much more then m-me!" You'd sob.

"I DONT WANT MORE! I WANT YOU!"

She'd cry out, clinging to you.

The both of you standing there for several moments, staring at each other as the girl held back her sobs.

"You... Your my best friend (Y/N)... Your the only person I've ever wanted... you... you mean everything to me (Y/N)..."

She spoke softly, staring into your tear stained eyes.

You'd grasp onto her, the two of you holding each other close as you broke down in tears.

You just clung to each other, the two of you breaking into tears, clinging to each other as you broke down, your only comfort being each other's embrace.

Youd sit there, holding each other for what felt like hours, unable to do anything but cry and sob, desperately holding onto each other.

But eventually you would both settle, eyes running dry as you held each other, both of you just leaning into each other's shoulder, holding each other.

Time would pass in a blink, both of you leaning on each other before you'd finally pull back, sluggishly leaning back as you rubbed your eyes.

Silence would ring out for several minutes as you wiped your eyes, doing your best to compose yourself, neither of you really looking at each other as you sat there.

"... Did..." you'd wipe your nose, still unable to look at her.

"Did you mean what you said... about... you know..."

You asked her, unwilling to put it into words lest she utterly deny it.

Octavia would sniffle wiping her nose, straightening herself as she reached out, cupping your cheek.

"I did. And I still do..."

She'd lean in, kissing your cheek.

"You... you mean so much to me... please don't ever, ever say your nothing."

She'd turn you to her, looking into your eyes.

"You mean so, so much to us (Y/N). To me. To me and my parents. Your... your a part of us. A part of our family."

She'd nuzzle her nose against your own.

"Your the best (Y/N). No matter what that bitch may say or think. You deserve all the love and attention in the universe."

She'd hold you close as you broke into giggles, the two of you quickly breaking into laughter as you held each other close.

After several minutes of laughter you'd settle, humming softly as you pulled back, the both of you staring into each other's eyes.

"... Thank you... Octavia..."

You spoke softly, looking into her eyes.

Octavia just smiled, nuzzling you.

"You deserve it. That and so... so much more."

She'd reach out, pulling you into a hug, you happily hugging her back, the girl gently nibbling at the feathers around your face.

It was a simple, but intimate act between Goetia.

Preening. A simple, delightful act between two demons that truly cared for each other..

You'd giggle as she nipped and nibbled on your feathers.

Eventually you'd pull back, giggle as she smiled at you.

You both sat there for a long moment before humming.

"Thanks O. You... Your really the best thing that ever happened to me."

You'd sit alone for another moment before she'd smile.

Getting up, she'd extend a hand, smiling down at you.

"Let's go, (Y/N)... Let's get out of here. You need a shower."

You'd stare at her hand for several moments before a gentle smile crossed your face, breaking into giggles.

Taking her hand she'd pull you up, smiling warmly as you pulled on her hand.

You'd stumble slightly, sniffling as you looked you her, the girl holding you close.

"Thanks O..." You spoke softly, leaning your head on her shoulder.

Octavia just smiled, moving forwards as she held you, the both of you walking into her manor.

Your home. The girl embraced you completely.

The young lady loving you unconditionally.

[marveloustimestwo]

Hii :))) please can I request some general headcanons for platonic tony stark? Sorry I don't have anything specific

Hello :) I'm always happy to do general headcanons for characters, so thanks for sending this in!

Warnings: Yandere themes, talk of Tony's daddy issues

I can see Tony getting attached in a few ways.

The easiest way this could happen is if you were his biological child, especially if you came from his Playboy days and he only found out a few years after you were born.

Tony can admit that he never had a good relationship with his father. The man had always been away for work, and even when he was home, he was cold and affectionless.

So in a case where he suddenly figures out that he has a kid whom he didn't have the chance to meet for several years, he will heavily overcompensate.

He never wants to be like his own dad and the fact that he wasn't around for the first few years will have him scrambling to make up for that.

Of course, the easiest way for Tony to do that initially is with his money. Depending on how old you are, he'll get you gifts that range from toys to books to super fancy computers.

And when he finds out what some of your interests are, you'll have far more than you would need to entertain those interests.

If you're an artist, you'll have all that you could need for your medium, such as incredibly expensive paint sets or the most expensive drawing tablet he could find.

If you enjoy reading, you'll have the most extensive library you could ever need, all right at your fingertips.

And Tony would be especially ecstatic if you were interested in science.

He wouldn't be like his father. Whatever interest you have, he'll be more than happy to learn about it just so he can spend time with you.

All those years without him won't mean much now that he's around all the time.

But if you take after him and are especially smart in science/robotics, you will not have a moment of peace.

He already spends hours in his lab, battling insomnia and nightmares, but you make it better. Now, working isn't just something to distract him, it's also something to bond with you over.

Even if the specific branch of science you're interested in is something he doesn't know yet, he would have no problem learning it.

Tony would happily spend hours in the lab with you, either building things with you or doing his own thing while you worked on your own interests.

The only things that would pull him out of it would be Pepper or Jarvis, who would have to remind him to take care of his own and your physical needs.

In that sort of situation, Tony would not take being separated from you again lightly. If your mother has issues with the way he interacts with you, he would have no problem trying to get full custody.

It would be rather easy considering he's filthy rich, but he'd still rather you have your mother in your life.

Another way I could see him becoming like this is obviously with Pepper.

It'd be like a dampened-down version of the last scenario. He'd want to be better than his father now that he has you, except unlike the previous scenario, he has all the time in the world to do it.

Especially since Pepper can and will keep him in check, so he won't overdo it completely. Tony just sees it as him screwing over his father and showing him that it can be done right.

Thirdly, while I think this is a little more unlikely, you could also be someone younger Tony ends up taking under his wing.

Especially if you're a young hero. If you work around or closely with the Avengers in some way, and he finds out that you're in your teens or early twenties, he will do his best to make sure you don't end up like him.

Tony has a lot of guilt, trauma, and PTSD from being a hero. He can recognize he was extremely arrogant when he was younger, and he didn't take things as seriously as he should have.

If he could save you from all of that, he would in a heartbeat.

Plotonically, Tony will try to do everything he can to make you feel loved. He refuses to be like his father. No matter what, you will know exactly how much he would do for you.

[raggedypina]

First let me just amend you on how AMAZING this au is... this one is truly gonna be a comfort one for me just due to the fact that connections and realationships are and the for front and I eat that stuff up along with art going along with a song is just right up my ally can't wait to see me

I have a couple of questions...

Ik you said you didn't want the bunnydoll to be the for front of the au but I gotta ask. Do you think it's possible that jax and rags would get back together (in the back of my head ik they won't). And if they don't is it possible that they could still build their relationship in a plotonic way???

What Kinger's view on how the band is? Dose he belive that theres hope or is on the same boat with jax thinking that the band is at it's end.

What everyone favorite song 👉🏾👈🏾

Again truly love this AU and my brain is just pouring out head canons lol. 💚

Firstly… THANK YOU!!! 🥹😭

I spent solid minutes simply staring at this message with tears in my eyes, but I don't want to be dramatic, so let's go…

1.I really just don't want the AU to be 100% bunnydoll, I want to make room for other ships like Zooble and Gangle or Kinger and Queenie (Examples of course), as well as focusing individually on each character before delving into the ship itself. As for the possibility of them getting back together…it depends entirely on how the band is doing, and mainly, whether the main reason for them breaking up has been resolved or discussed. Platonic relationships are easier, and one path would lead to another thinking that maybe they still have feelings for each other *wink*

2. Since Queenie left Kinger doesn't really "think" about much, he rarely even attends rehearsals and shows. But in the context of the question, he believes it's just a bad phase

Now with the favorite song!! (Just to make it clear that these songs are what they consider their favorites, it doesn't necessarily mean that they identify with each other)

Gangle - "Feel Special" (Twice)

Zooble - "Misery Business" (Paramore)

Kinger - "The Happy Wanderer" (Frank Weir)

Jax - "Freaks" (Surf Curse)

Ragatha - "Somewhere Only We Know" (Keane)

Pomni - "Bananaphone" (Raffi) *She doesn't have very good song references, give her a break*

Again, thank you very much!!! I'll do my best to make it as interesting as possible!!!

[nickquin]

“You see me now tonight. You see me more than ever-“ “she does not sound like that”

Happiest of late birthdays to the lovely @mayahawkins !!! You are one of the sweetest and kindest people I know and every convo we have is amazing. I know you are a bit of a slump with gifs but I love seeing each set you produce and hope you are having a lovely day !!!!!

[gatorbites-imagines]

Can you please do a plotonic Alien Reader x Damian Wayne?

Basically, the reader is from an alien race that is known to raise their hatchlings in groups/ colonies.

Reader just takes one look at Damian after joining the heros, and was like "yep, that's also my child now."

Reader just helping Damian with his sensory issues and being a somewhat emotionally available parental figure.

- 🦊

Platonic Damian Wayne and Alien male reader

Headcanons

Imagine the reader and Batman hissing at each other like angry alleyway cats because they both are adoption addicts, and have spotted an orphan at the same time.

You were from an alien species. You were reptilian in appearance, think Argonian from Skyrim, except you are taller and more built, have 6 eyes, 4 arms, and a long flexible tail.

What makes your species special is the fact that most have an elemental power of some sort. For most it isn’t very powerful, but for you it was strong enough that you were able to defend your home planet from invaders, singlehandedly.

Where most of your kind will only control one element, you are able to use all of them, and even able to do things like warp energy, magnetism, liquification, and more.

Your power is what has helped you defend not only your home planet, but earth, and the universe. But it has also hindered your ability to take part in your people’s tradition of raising children in colonies. You are so powerful your duty is to protect the universe, meaning you are far away from home most of the time, which leaves you feeling like you’re missing something most of the time.

Your species feels a deep urge to love and raise young, and things like adoption is very common as all kids are pretty much mutual ground, though children tend to have one or two people they prefer more than everyone else.

This also leads to a lot of outside adoption, as your people see children as most precious and think all children deserve a great future and good childhood.

When you met the justice league for the first time, it was because you were hunting down the current big bad who had wanted to destroy your home planet, and they fled to earth. The league didn’t even have time to take the guy down before you had smited him with lightning strong enough to make Zeus sweat.

It took a while for the league not to think you were a threat, but thanks to the green lanterns having worked with your people before, they were able to explain who you were and act as translators.

Your species doesn’t possess vocal cords the same way earthlings do, resulting you in having to use some of your powers to allow you to speak in a way they understand. There are new heroes on your home planet, so you are able to stay on earth.

Your people see it as a trading of information, like an exchange student program, as they are curious about the league and the league is curious about you, since you are rumored to be very powerful throughout the galaxy.

When you see all the young heroes for the first time you almost have a heart attack, because why would these adults allow the young ones to go out and put themselves in danger like that.

It took some explaining from Hal and other Lanterns to explain the cultural difference, and though you aren’t a fan you accept that its part of their culture, and that children aren’t coddled here like on your home planet.

On another note, you become quite popular with the younger heroes, since you always try to help out and treat them as equals.

The first time you meet Damian the parental part in your biology immediately starts chanting and making noise, pointing at Damian, and yelling “our child now”.

Of course, you can smell he’s batman’s offspring, but you notice that Batman doesn’t meet the standards for a guardian, and before anyone knows it you’ve scooped Damian up and declared him yours.

This of course doesn’t happen one day to the next, it takes a lot of bonding and getting to know each other. Damian has no idea what to do with the fact that you are so willing to be emotionally available to him, as he’s never truly had that.

 When you learn that Damian is autistic (my hc), you immediately start researching autism in humans and how it affects them and their daily lives. This becomes very useful in your everyday life too of course, but it’s mainly for Damian.

Damian liking animals so much probably helps too, since you look like a lizard for the most part. You liking to sunbathe for warmth results in Damian joining you even though he doesn’t need it, and that becomes your spot. That’s when he opens up to you about his issues after a while, and you grow closer.

You start carrying noise cancelling headphones around when you learn Damian gets sensory overload, and use your powers to block out noise or light if it becomes too much for him.

With Damian comes the rest of the bats, and you are more than ready to adopt all of them. You don’t even comprehend how this isn’t normal for earthlings, so when you start to refer to them as your pod or colony, you don’t notice the confused looks you get.

Cue confrontation with Bruce, though to everyone else it just looks like two feral cats puffing up at each other and hissing a bit. You end up having to get outside help to figure it out, and after that you two figure it out.

Some of your teammates and the batkids definitely make fun of you guys for being coparents. I can imagine Jason calling himself a child of divorced parents even though you and Bruce are nothing but friends.

But even though you’ve pretty much accepted all of them into your colony, Damian is still your baby. He’s just young and small and you want to protect him, even though he hates being babied. Damian will never say it out loud but he likes being someone’s number one no matter what.

[plus-ultra-oneshots]

-Requests Rules-

Hello all~! Please call me Puo! (or PUO lol)

Imma put together a few rules for Requests/Asks!:

**Please do be aware that I probably won’t be very responsive/active on this blog! I write a lot, but I have several fanfics in the works and they take precedence!

If you wanna read my fanfics, please check out my Pages on Fanfiction, Wattpad and AO3 (I write a lot for both Fairy Tail and My Hero Academia):

-Wattpad -Fanfiction -Archive Of Our Own

Also, please be aware that I’m a pretty slow writer, and I’m busy a lot with my fanfics, with college, and with my art commissions and trades. >w<

-- What I Will Write For --

One Shots/ Imagines/ Headcannons

Fluff (I love fluff)

Yuri/ Yaoi / Straight / All other Pairings

Blood/ Gore / Violence

Reader X whoever

Cannon X Cannon pairings

Light NSFW/Smut (though sparingly... I will keep things suggestive, and never explicit!)

Plotonic/Friendship/BrOTP stuff

Sensitive Stuff (Depression, self-harm, etc) - Please Inquire, and respect my right to refuse.

All others- Inquire.

-- What I Will NOT Write For --

(I will never reply to any asks that fall under these)

Rape/Non Con

Incest

Pedophila

Cockwarming

Hardcore NSFW

Super freakin’ weird kink/fetish stuff (foot, fat fetish- for example)

-- Characters I Can Write For --

All of Class 1-A

Shota Aizawa/Eraserhead

Toshinori Yagi/ All Might

Naomasa Tsukauchi

Hizashi Yamada/ Present Mic

Nemuri Kayama/ Midnight

Keigo Takami/ Hawks

Taishiro Toyomitsu / Fatgum

The Big Three

Most of Class 1-B

LOV

... TBH, I can write for pretty much anybody with relative accuracy (I believe). These in the list are just the ones I could come up with off the top of my head. I don’t have any characters that are blacklisted, so feel free to inquire!

I am mostly caught up on the My Hero Academia Manga, and I have read near all of the Vigilantes Manga too.... I will attempt to not include spoilers!

-- A Note on Stealing/Copy-pasting --

These are all for your enjoyment of course, and I love that you guys enjoy the stuff I write and really get into the stories and the scenarios! And I love that you guys might get your creative juices flowing by reading my stuff! I would like nothing more than to be an inspiration for you, and to maybe give you a bit of enjoyment too!

That being said though- DO NOT STEAL THESE FROM ME! NOT THE STORIES, NOT THE QUIRKS I COME UP WITH- NOTHING!

I have had people steal my oneshots/imagines before (quite blatantly at that... copying and pasting), and there is nothing more aggravating, nor disrespectful. These are for you to enjoy reading, and I do hope you enjoy them! But it is so terribly hurtful to see ones work stolen with little a care! Please do not do it!

Thank you~!

.:+:.

MasterList Here

[heroesarelife]

Hi yaaa! Could you write some headcanons for Bakuspuad having a new mother friend who had a healing quirk ? I'm craving for some Bakuspuad plotonic fluff! Thank you if you can write and It's allright if you don't want to write ! Take care

*sneaks into my wife’s blog* ~ Undead Raven from @kinkyacademia

Having a new mom friend in the group was certainly a change for the five friends, of course, they all loved her dearly, even the grumpy, foul-mouthed Katsuki had come to appreciate her company as he had for the others in his squad.

For Sero, it was a refreshing change, she was similar to him, constantly worrying about the group and their well-being but unlike him, she was able to heal some of the injuries that would sometimes just so happen to appear.

Ashido had a great time going shopping with her and Denki and the three of them entertained Eijiro’s sense of fashion. Though it was quite frankly disastrous, his bright red crocs standing out like a blinding white-light for those of you that had a good sense of fashion.

Denki’s perverted tendencies tended to disappear in her line of sight in fear of being scolded, or worse, revealed to the girls and left to fend for himself. Other than that, the two of them share some good times by sitting together and reading through articles on their phones while he “charges” himself in a energy outlet.

With a healing quirk, she was able to backup the others in training and keep them in fighting shape. And in turn, they helped her develop a fighting style that maintained her safety and made sure she wasn’t left defenseless on the job.

It became an habit for everyone in the group to regulate their health once she became a member of the bakusquad. Eating right, making sure that their diets matched their quirks needs, getting enough sleep. Anything that could affect their health really because if they weren’t on top of it, she would be. And they wouldn’t want that.

Movie nights are either a disaster or a feather-ridden war zone. There is no in-between, though she often had to play medic for the pillow related injuries that they (Bakugou) caused to each other. And due to her neutral position in the movie night pillow fight, Eijiro always remained close by so that he could harden and keep the medic safe.

Not everyone in the Bakusquad is as good in the kitchen as Bakugou himself so she is often hovering and making sure no one sets anything on fire or cuts themselves. As a bonus for the others, she always limit how much spice and curry Katsuki is allowed to put in a shared dish, keeping things mild at best.

If by chance she’s the only one in the kitchen with Katsuki, the spices are broken out heavily, being the only other member of the bakusquad that can handle a decent amount of spice, it became a way for the two of them to get along best. Whoever could eat the most won and first to reach for a glass of water had to do one thing the winner said. No limits. Thankfully the two of them know not to push each other too much.

The Bakusquad has only ever seen her angry once before and each member agreed that she is definitely a force best left alone during those times. Her favourite foods are often nudged towards her from a distance and the others shove a complaining Katsuki at her with said offerings.

[aartisenblog]

  GET THIS BOOK

Author:

John Crocker & Paul G. Murray

Published in: John Wiley & Sons Release Year: 2003 ISBN: 0-470-84475-2 Pages: 393 Edition: First Edition File Size: 4 MB File Type: pdf Language: English

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Description of Molecular Biology in Cellular Pathology

Since the publication of the original edition of Molecular Biology in Cellular Pathology book, there have been rapid advances in our understanding of disease, mainly as a result of the impetus provided by some of the newer technologies. In particular, the rapidly developing fields of genomics and proteomics are enabling an understanding of gene expression both at the mRNA and protein level on a global scale (i.e. the whole transcriptome or proteome) not previously imaginable. Whereas gene expression studies in pathology have frequently relied purely on immunohistochemistry and in situ hybridisation, in their own right still immensely invaluable procedures, they could only essentially give information on a single gene in a single experiment. Now information on expression from the whole of the genome can be assessed in a single experiment. Proteomics, in particular, is providing the tools not only to examine global protein expression, but also to dissect protein function, through the development of approaches to study protein activity. Likewise, in genetics, there is an impending revolution. Comparative genomic hybridisation, for a long time a difficult alternative to conventional cytogenetics, will blossom with the advent of array approaches to, allowing high resolution mapping of chromosomal changes across the whole genome without the need for difficult interpretation of chromosome morphology. The key question is to what extent these developments will impact on diagnostic pathology in the future. The polymerase chain reaction was heralded years ago with the view that its introduction into routine diagnostic pathology practice was only a matter of time. Events have not proved this assumption correct; although the polymerase chain reaction does have applications in routine pathology it has not impacted directly in a significant way on routine histopathology. It is the authors’ view that the same will not be true of the newer technologies. At the very least these newer approaches may identify a whole host of disease-specific markers for use in conventional assays for disease. At the other end of the spectrum, they may change forever the morphological assessment of disease to be substituted by an entirely objective set of array data providing detailed information on chromosome changes and global gene expression. We hope that this new edition will give some insights into some of the developments in molecular biology that provide us not only with immense opportunities for the future but also with considerable challenges. We would particularly like to thank all those who have contributed to this text and also our families, to whom we are of course deeply indebted for allowing us to pursue this project, often at their expense. Mrs Ruth Fry supplied excellent secretarial support.

Content of Molecular Biology in Cellular Pathology

1 Blotting Techniques: Methodology and Applications 1 Fiona Watson and C. Simon Herrington 1.1 Introduction 1 1.2 Blotting techniques 1 1.3 References 15 2 In-situ Hybridisation in Histopathology 19 Gerald Niedobitek and Hermann Herbst 2.1 Introduction 19 2.2 Experimental conditions 20 2.3 Probes and labels 23 2.4 Controls and pitfalls 27 2.5 Double-labelling 29 2.6 Increasing the sensitivity of ISH 31 2.7 What we do in our laboratories 33 2.8 Applications of ISH: examples 35 2.9 Perspective 39 2.10 References 40 3 DNA Flow Cytometry 49 M.G. Ormerod 3.1 Introduction 49 3.2 Definitions and terms 49 3.3 Dye used for DNA analysis 50 3.4 Sample preparation for DNA analysis 52 3.5 Analysis of the DNA histogram 53 3.6 Quality control 53 3.7 Computer analysis of the DNA histogram 55 3.8 Multiparametric measurement 57 3.9 Acknowledgements 59 3.10 References 59 4 Interphase Cytogenetics 61 Sara A. Dyer and Jonathan J. Waters 4.1 Introduction 61 4.2 Interphase cytogenetics 62 4.3 Applications 67 4.4 Conclusion 76 4.5 References 77 5 Oncogenes 79 Fiona Macdonald 5.1 Introduction 79 5.2 Identification of the oncogenes 79 5.3 Functions of the proto-oncogenes 80 5.4 Mechanism of oncogene activation 89 5.5 Oncogenes in colorectal cancer 91 5.6 Oncogenes in breast cancer 94 5.7 Oncogenes in lung cancer 95 5.8 Oncogenes in haematological malignancies 96 5.9 Other cancers 99 5.10 Conclusion 100 5.11 References 100 6 Molecular and Immunological Aspects of Cell Proliferation 105 Karl Baumforth and John Crocker 6.1 The cell cycle and its importance in clinical pathology 105 6.2 Molecular control of the cell cycle 108 6.3 Cell cycle control 111 6.4 The cell cycle and cancer 112 6.5 Immunocytochemical markers of proliferating cells 115 6.6 References 133 6.7 Further Reading 135 7 Interphase Nucleolar Organiser Regions in Tumour Pathology 137 Massimo Derenzini, Davide Trer ́e, Marie-Fran ̧coise O’Donohue and Dominique Ploton 7.1 Introduction 137 7.2 The AgNORs 138 7.3 NOR silver-staining 142 7.4 Quantitative AgNOR analysis 145 7.5 AgNORs as a parameter of the level of cell proliferation 146 7.6 Application of the AgNOR technique to tumour pathology 147 7.7 What future for AgNORs in tumour pathology? 151 7.8 References 152 8 Apoptosis and Cell Senescence 153 Lee B. Jordan and David J. Harrison 8.1 Introduction 153 8.2 Apoptosis 153 8.3 Cell senescence 174 8.4 Summary 178 8.5 References 179 9 The Polymerase Chain Reaction 193 Timothy Diss 9.1 Introduction 193 9.2 Principles 194 9.3 Analysis of products 197 9.4 RT-PCR 199 9.5 Quantitative PCR 200 9.6 DNA and RNA extraction 200 9.7 Correlation of the PCR with morphology 201 9.8 Problems 202 9.9 Applications 202 9.10 Diagnostic applications 203 9.11 Infectious diseases 209 9.12 Identity 209 9.13 The future 210 9.14 References 210 9.15 Online information 212 10 Laser Capture Microdissection: Techniques and Applications in the Molecular Analysis of the Cancer Cell 213 Amanda Dutton, Victor Lopes and Paul G. Murray 10.1 Introduction 213 10.2 The principle of LCM 214 10.3 Technical considerations 216 10.4 Advantages and disadvantages of LCM 217 10.5 Applications of LCM 222 10.6 Future perspectives 229 10.7 Acknowledgements 229 10.8 References 229 11 The In-situ Polymerase Chain Reaction 233 John J. O’Leary, Cara Martin and Orla Sheils 11.1 Introduction 233 11.2 Overview of the methodology 234 11.3 In-cell PCR technologies 235 11.4 In-cell amplification of DNA 238 11.5 Detection of amplicons 242 11.6 Reaction, tissue and detection controls for use with in-cell DNA PCR assays 243 11.7 In-cell RNA amplification 244 11.8 Problems encountered with in-cell PCR amplification 246 11.9 Amplicon diffusion and back diffusion 247 11.10 Future work with in-cell PCR-based assays 247 11.11 References 249 12 TaqMan Technology and Real-Time Polymerase Chain Reaction 251 John J. O’Leary, Orla Sheils, Cara Martin, and Aoife Crowley 12.1 Introduction 251 12.2 Probe technologies 252 12.3 TaqMan probe and chemistry (first generation) 254 12.4 Second generation TaqMan probes 256 12.5 Hybridisation 258 12.6 TaqMan PCR conditions 259 12.7 Standards for quantitative PCR 260 12.8 Interpretation of results 261 12.9 End-point detection 262 12.10 Real-time detection 263 12.11 Relative quantitation 263 12.12 Reference genes 264 12.13 Specific TaqMan PCR applications 265 12.14 References 268 13 Gene Expression Analysis Using Microarrays 269 Sophie E. Wildsmith and Fiona J. Spence 13.1 Introduction 269 13.2 Microarray experiments 269 13.3 Data analysis 273 13.4 Recent examples of microarray applications 284 13.5 Conclusions 284 13.6 Acknowledgements 284 13.7 References 284 13.8 Further Reading 286 13.9 Useful websites 286 14 Comparative Genomic Hybridisation in Pathology 287 Marjan M. Weiss, Mario A.J.A. Hermsen, Antoine Snijders, Horst Buerger, Werner Boecker, Ernst J. Kuipers, Paul J. van Diest and Gerrit A. Meijer 14.1 Introduction 287 14.2 Technique 289 14.3 Data analysis 292 14.4 Applications 293 14.5 Clinical applications 299 14.6 Screening for chromosomal abnormalities in fetal and neonatal genomes 299 14.7 Future perspectives 300 14.8 Acknowledgements 301 14.9 References 301 15 DNA Sequencing and the Human Genome Project 307 Philip Bennett 15.1 Introduction 307 15.2 DNA sequencing: the basics 308 15.3 Applications of DNA sequencing 318 15.4 The Human Genome Project 320 15.5 References 327 15.6 Further Reading 327 15.7 Useful websites 328 16 Monoclonal Antibodies: The Generation and Application of ‘Tools of the Trade’ Within Biomedical Science 329 Paul N. Nelson, S. Jane Astley and Philip Warren 16.1 Introduction 329 16.2 Antibodies and antigens 331 16.3 Polyclonal antibodies 332 16.4 Monoclonal antibody development 333 16.5 Monoclonal antibody variants 338 16.6 Monoclonal antibody applications 341 16.7 Therapy 345 16.8 Specific applications 346 16.9 Conclusions 347 16.10 Acknowledgements 347 16.11 References 347 17 Proteomics 351 Kathryn Lilley, Azam Razzaq and Michael J. Deery 17.1 Introduction 351 17.2 Definitions and applications 352 17.3 Stages in proteome analysis 352 17.4 Future directions 368 17.5 References 368 Index 371

[lbcreations-blog]

Husker, Charlie, and angel (seperate) with a teen reader who’s often found hanging around the hotel, not because they’re a sinner looking to rehabilitate (they’re hellborn) but just cuz they’re bored and want some entertainment. Overtime husker, Charlie and angel have become older familial figures and really close to reader. So what if reader comes to them one day and admits the real reason they’re always at the hotel is bc they’re being sexually abused at home? Sorry if you don’t feel comfortable with this, I’m honestly feeling a but uncomfortable requesting it but the comfort is much needed

If ya don’t wanna write this it’s ok just let me know pls

Auther note to requester: Of course, I'll write this, I also need comfort on this stuff, so yea. I had a bit of a writers block, and this is also not my best work, which I apologize for. Plus, I apologize for this coming out so late.

I have no idea why husks section is so short😭

All plotonic

Gn reader

The reader is shorter than Angel and Charlie and the same height as husk, btw

TW: slight descriptions of injuries and mentions of signs of struggle

Masterlist

No one should hurt someone like this

You loved hanging around at the hotel. You enjoying the chaos of the residents

You were there basically every day

You got very close to the bartender at the hotel

Husk was like an acutel father to you

He would always talk to you. Sometimes, he would show you magic tricks, and you loved it

One day, you seemed so sad

So Husk decided to wait until everyone's out of the lobby and ask what's up

Once, you had the courage to tell him he got angry at the ones who were supposed to raise you and nurture you.

But before he would do anything to the ones at your so-called 'home' he would comfort you

It might be a bit awkward, but he would do his best

He might even ask Charlie to let you stay at the hotel as an official resident

After comforting you, he would wait for the day he beats the ones who hurt you assess

Charlie was confused as to why you would hang around at the hotel for you being hellborn like her

But she let you stay thinking that even you, a hellborn, was supporting her hotel

After a while of being at the hotel, you and Charlie bonded quite nicely

She was like a super cool, bubbly, older sister to you

Compared to basically everyone in hell, you generally vibed with her, for some reason

Now, one day, you just weren't at the hotel

And then the next day

And the one after that

Now Charlie was worried, so she got the forms that tells where everybody lives and makes her way over to your adobe

Charlie walked up to your door and knocked the knocker three times.

No answer

After waiting for two minutes, she knocked three times once again.

Again no answer

She knocked with the knocker against the door one more time, but to no avail.

No answer

Charlie knew you were in your house she could sense your aroa, and your aroa felt hurt, so she knew she knew that had to do one thing and one thing only.

Go into your house without your permission.

She had to go in to make sure you were ok. So she tried to go into your house. But the door was locked. So she unlocked the door with her powers and stepped inside, and goodness, the place looks terrible. The couch was flipped in the lounge, and other signs off struggle was obvious.

Charlie walked around the house carefully in case someone else was lurking in the house. After a while, she found herself in front of a door with your name on it. This was definitely your room. She opened your door with caution, and...

There was you.

On the floor.

Sitting there with your hands covering your face.

There were bruises on your body, and you were quietly sobbing.

Charlie gasped upon the sight that was displayed in front of her. You looked up at her. You looked, so... terrified. And Charlie hated it so much. She quickly rushed to your side and softly hugged you so as not to hurt you more.

"Hey, hey," Charlie said in a soft voice. "Are you badly hurt? And do you know who hurt you?"

"I-" you sobbed softly.

"Hey. Hey, it's OK, it's OK, take your time," Charlie told you in the most comforting voice she could muster.

"The, ones I live with, did this to me" you told her in between sniffles.

Charlie was angered on this fact but stayed with you and tried to keep her calm. All she knew was that she would make sure those assholes would be dealt with, biological or not, no one hurts her younger sibling.

Angel Dust, the porn star

A tricky fella per-say

How did you become friends? It's hard to say

But for me and everyone else, you are like 12 - 14 in his version, and since you are oh so young, he doesn't talk about that stuff around you, like he might cuse but that's cause everyone does that

Anyway, now you both are pretty close, maybe you both like to dress up to together or smthn

After a while, you kinda start feeling like Angel is your older brother like yall have so much fun together

So since you veiw him as such, you felt as if you should share something you think he'll know about, even though it's supposed to be a secret

You softly knocked on Angel's bedroom door. "Hey Angel, it's me, Y/N," the door opened to show Angel. "Oh Heya kid, how'ya been?" Angel asked you. "Oh, ok, I guess... hey, uhm, I wanna talk to you in your room." You told the sinner with an uncomfortable face.

"Uh, alright com'n in kid," he said, moving out of the way off the door. You both sat down on his couch. "So what's been troubling, you kid?" Angel asked you. "Uhm, well, I'll just show you." You said as you brought your turtleneck down. Once Angel realized what he was seeing, he was shocked, angry, and just sad.

There was a hikey on your neck. "Uhm, Angel, do you know what this is?" You asked him, but instead of getting a response back.

You got a hug.

A very nice and warm one.

Sooner or later, Angel was gonna kill those basstards that hurt you.

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