#Microbial Testing

[this-is-my-main-i-follow-from]

Chef WK, lead charcuterie specialist in Alberta Canada

Table of contents

1. Control Program Requirements for Fermented Meat Products

2. Facility and Equipment Requirements

3. Starter Culture

4. Chemical Acidification

5. Water Activity Critical Limits

6. Time and Temperature for Fermented Products

7. Fermentation Done at a Constant Temperature

8. Examples of Degree-hours at constant room temperatures

9. Fermentation Done at Different Temperatures

10. Fermentation done at Different temperatures

11. What happens if fermentation fails to hit critical limit?

12. E. coli and Salmonella Control in Fermented Sausages

13. Options for E. coli validation

14. Option1; Heating

15. Option 2; pH, heating, holding, diameter

16. Safety and consistency

Control Program Requirements for Fermented Meat Products

The producer must have a program in place to assess the incoming product. This program should outline specifications for the incoming ingredients. This may include criteria including receiving temperature, farm/ supplier, lot code or packed on date, species/cut etc.

2. Facility and Equipment Requirements

Equipment used in the fermentation process must be included in the operator's prerequisite control programs. These must include the following elements:

Temperature in the fermentation, drying and smoking chambers must be uniform and controlled to prevent any fluctuation that could impact on the safety of the final product.

Fermentation, drying and smoking chambers must be equipped with a shatter resistant indicating thermometer, (or equivalent), with graduations of 1°C or less. If mercury thermometers are used, their mercury columns must be free from separations. All thermometers must be located such that they can be easily read.

Fermentation and smoking chambers must be equipped with a recording thermometer for determining degree-hours calculations in a reliable manner. Recording thermometers are also preferable in drying and aging rooms but, in these rooms, it may be sufficient to read and record the temperatures 2 times a day.

Drying and aging rooms must be equipped with humidity recorders in order to prevent uncontrolled fluctuations of the relative humidity. The only alternative to an automatic humidity recorder in these rooms would be for the company to manually monitor and record ambient humidity twice a day (morning and afternoon) every day with a properly calibrated portable humidity recorder.

For routine monitoring, accurate measurement electronic pH meters (± 0.05 units) should be employed. It is important that the manufacturer's instructions for use, maintenance and calibration of the instrument as well as recommended sample preparation and testing be followed.

When the aw of a product is a critical limit set out in the HACCP plan for a meat product, accurate measurement devices must be employed. It is important that the manufacturer's instructions for use, maintenance and calibration of the instrument be followed.

3. Starter Culture

The operator must use a CFIA approved starter culture. This includes Freeze-dried commercially available culture as well as back-slopping (use of previously successful fermented meat used to inoculate a new batch). When performing back-slopping, the operator must have a control program in place to prevent the transmission of pathogens from when using the inoculum from a previous batch to initiate the fermentation process of a new batch. These must include:

The storage temperature must be maintained at 4°C or less and a pH of 5.3 or less.

Samples for microbiological analysis must be taken to ensure that the process is in line with the specifications.

The frequency of sampling is to be adjusted according to compliance to specifications.

Any batch of inoculum which has a pH greater than 5.3 must be analysed to detect at least Staphylococcus aureus. Only upon satisfactory results will this inoculum be permitted for use in back slopping.

This can be an expensive and a time exhaustive process and is generally avoided due to food safety concerns. AHS does not allow back-slopping.

[Chef WK was in communication with the U of A to get his method, a starter mix, studied.]

4. Chemical Acidification

If product is chemically acidified by addition of citric acid, glucono-delta-lactone or another chemical agent approved for this purpose, controls must be in place and records kept to ensure that a pH of 5.3 or lower is achieved by the end of the fermentation process. These acids are encapsulated in different coatings that melt at specific temperatures, which then release the powdered acids into the meat batter and directly chemically acidulate the protein.

Summer sausage is a very common chemically acidified product. The flavor profile tends to be monotone and lacking depth. 

5. Water Activity Critical Limits

The aw may be reduced by adding solutes (salt, sugar) or removing moisture.

Approximate minimum levels of aw (if considered alone) for the growth of:

molds: 0.61 to 0.96

yeasts: 0.62 to 0.90

bacteria: 0.86 to 0.97

Clostridium botulinum: 0.95 to 0.97

Clostridium perfringens: 0.95

Enterobacteriaceae: 0.94 to 0.97

Pseudomonas fluorescens: 0.97

Salmonella: 0.92 - 0.95

Staphylococcus aureus: 0.86

parasites: Trichinella spiralis will survive at an aw of 0.93 but is destroyed at an aw of 0.85 or less.

The above levels are based on the absence of other inhibitory effects such as nitrite, competitive growth, sub-optimum temperatures, etc., which may be present in meat products. In normal conditions, Staphylococcus aureus enterotoxins are not produced below aw 0.86, although in vacuum packed products this is unlikely below aw 0.89.

6. Time and Temperature for Fermented Products

Certain strains of the bacteria Staphylococcus aureus are capable of producing a highly heat stable toxin that causes illness in humans. Above a critical temperature of 15.6°C, Staphylococcus aureus multiplication and toxin production can take place. Once a pH of 5.3 is reached, Staphylococcus aureus multiplication and toxin production are stopped.

Degree-hours are the product of time as measured in hours at a particular temperature multiplied by the "degrees" measured in excess of 15.6°C (the critical temperature for growth of Staphylococcus aureus). Degree-hours are calculated for each temperature used in the process. The limitation of the number of degree-hours depends upon the highest temperature in the fermentation process prior to the time that a pH of 5.3 or less is attained.

The operator is encouraged to measure temperatures at the surface of the product. Where this is not possible, the operator should utilize fermentation room temperatures. The degree hour calculations are based on fermentation room temperatures. Temperature and humidity should be uniform throughout the fermentation room.

A process can be judged as acceptable provided the product consistently reaches a pH of 5.3 using:

fewer than 665 degree-hours when the highest fermentation temperature is less than 33°C;

fewer than 555 degree-hours when the highest fermentation temperature is between 33° and 37°C; and

fewer than 500 degree-hours when the highest fermentation temperature is greater than 37°C.

This means that as the temperature increases, the amount of time that you have available to reach 5.3 or under is shorter. The warmer the temperature, the sharper the log growth phase of bacteria, which equates to more overshoot in lactic acid production, faster.

8. Examples of Degree-hours at constant room temperatures

Example 1:

Fermentation room temperature is a constant 26°C. It takes 55 hours for the pH to reach 5.3.

Degrees above 15.6°C: 26°C - 15.6°C = 10.4°C Hours to reach pH of 5.3: 55 Degree-hours calculation: (10.4°C) x (55) = 572 degree-hours

The corresponding degree-hours limit (less than 33°C) is 665 degree-hours.

Conclusion: Example 1 meets the guideline because its degree-hours are less than the limit.

Example 2:

Fermentation room temperature is a constant 35°C. It takes 40 hours for the pH to reach 5.3.

Degrees above 15.6°C: 35°C - 15.6°C = 19.4°C Hours to reach pH of 5.3: 40 Degree-hours calculation: (19.4°C) x (40) = 776 degree-hours

The corresponding degree-hours limit (between 33 and 37°C) is 555 degree-hours.

Conclusion: Example 2 does not meet the guideline because its degree-hours exceed the limit

9. Fermentation Done at Different Temperatures

When the fermentation takes place at various temperatures, each temperature step in the process is analyzed for the number of degree-hours it contributes. The degree-hours limit for the entire fermentation process is based on the highest temperature reached during fermentation.

Example 1:

It takes 35 hours for product to reach a pH of 5.3 or less. Fermentation room temperature is 24°C for the first 10 hours, 30°C for second 10 hours and 35°C for the final 15 hours.

Step 1

Degrees above 15.6°C: 24°C - 15.6°C = 8.4°C Hours to reach pH of 5.3: 10 Degree-hours calculation: (8.4°C) x (10) = 84 degree-hours

Step 2

Degrees above 15.6°C: 30°C - 15.6°C = 14.4°C Hours to reach pH of 5.3: 10 Degree-hours calculation: (14.4°C) x (10) = 144 degree-hours

Step 3

Degrees above 15.6°C: 35°C - 15.6°C = 19.4°C Hours to reach pH of 5.3: 15 Degree-hours calculation: (19.4°C) x (15) = 291 degree-hours

Degree-hours calculation for the entire fermentation process = 84 + 144 + 291 = 519

The highest temperature reached = 35°C

The corresponding degree-hour limit = 555 (between 33°C and 37°C)Conclusion: Example 1 meets the guideline because its degree-hours are less than the limit.

10. Fermentation done at Different temperatures

Example 2:

It takes 38 hours for product to reach a pH of 5.3 or less. Fermentation room temperature is 24°C for the first 10 hours, 30°C for the second 10 hours and 37°C for the final 18 hours.

Step 1

Degrees above 15.6°C: 24°C - 15.6°C = 8.4°C Hours to reach pH of 5.3: 10 Degree-hours calculation: (8.4°C) x (10) = 84 degree-hours

Step 2

Degrees above 15.6°C: 30°C - 15.6°C = 14.4°C Hours to reach pH of 5.3: 10 Degree-hours calculation: (14.4°C) x (10) = 144 degree-hours

Step 3

Degrees above 15.6°C: 37°C - 15.6°C = 21.4°C Hours to reach pH of 5.3: 18 Degree-hours calculation: (21.4°C) x (18) = 385.2 degree-hours

Degree-hours calculation for the entire fermentation process = 84 + 144 + 385.2 = 613.2

The highest temperature reached = 37°C

The corresponding degree-hour limit = 555 (between 33°C and 37°C)

Conclusion: Example 2 does not meet the guidelines because its degree-hours exceed the limit.

11. What happens if fermentation fails to hit critical limit?

What happens if the batch takes longer than degree-hours allows? For restaurant level production, it's always safer to discard the product. The toxin that Staph. Aureus produces is heat stable and cannot be cooked to deactivate. In large facilities that produce substantial batches, the operator must notify the CFIA of each case where degree-hours limits have been exceeded. Such lots must be held and samples of product submitted for microbiological laboratory examination after the drying period has been completed. Analyses should be done for Staphylococcus aureus and its enterotoxin, and for principal pathogens, such as E. coli O157:H7, Salmonella, and Clostridium botulinum and Listeria monocytogenes.

If the bacteriological evaluation proves that there are fewer than 104 Staphylococcus aureus per gram and that no enterotoxin or other pathogens are detected, then the product may be sold provided that it is labelled as requiring refrigeration.

In the case of a Staphylococcus aureus level higher than 104 per gram with no enterotoxin present the product may be used in the production of a cooked product but only if the heating process achieves full lethality applicable to the meat product.

In the case where Staphylococcus aureus enterotoxin is detected in the product the product must be destroyed.

12. E. coli and Salmonella Control in Fermented Sausages

Business' that manufacture fermented sausages are required to control for verotoxinogenic E. coli including E. coli O157:H7 and Salmonella when they make this type of product. This includes:

establishments which use beef as an ingredient in a dry or semi-dry fermented meat sausage;

establishments which store or handle uncooked beef on site;

Establishments which do not use beef and do not obtain meat ingredients from establishments which handle beef are not currently required to use one of the five options for the control of E. coli O157:H7 in dry/semi-dry fermented sausages. 

Any processed RTE product containing beef or processed in a facility that also processed beef, must be subjected to a heat treatment step to control E. coli O157:H7. Heating to an internal temperature of 71°C for 15 seconds or other treatment to achieve a 5D reduction is necessary. This is a CFIA requirement and is not negotiable.

Uncooked air dried products produced as RTE, must meet shelf stable requirements as detailed for Fermented-Dry products.

13. Options for E. coli validation

Without lab testing, the two main methods of validation are with heat treating by either low temp and a long duration, or various hotter processing temperatures for a shorter timeframe.

A challenge study to validate a process can take 1 year and over $100,000!

14. Option1; Heating

15. Option 2; pH, heating, holding, diameter

16. Safety and consistency

The aw and pH values are critical in the control of pathogens as well as to ensure shelf-stability in all semi-dry and dry fermented meat products. Each batch must be tested for aw and/or pH in order to verify that the critical limits are met.

Although aw measurement is mandatory only for shelf stable products, it is strongly recommended that the producer determine the aw values achieved for each product type they manufacture and for each product. Once this has been established, frequent regular checks should be made to ensure consistency. In the U.S., they rely on moisture to protein ratio and have set targets. This lab-tested value is a direct correlation of the % water to % meat protein and not aw. This gives more consistency to common names. For example, to legally call a product "jerky" it must have a MPR of 0.75:1 or lower. Remember your ABCs:

Always be compliant. 

-AND-

Documentation or it didn't happen.

(tags)

Charcuterie,Fermented Meat,Food Safety,Starter Culture,Chemical Acidification,Water Activity,Fermentation Process,Degree-Hours Method,Foodborne Pathogens,Meat Processing Guidelines,Chef WK Alberta Canada,Food Industry Standards,pH Critical Limits,Thermal Processing,Food Preservation,Food Microbiology,Sausage Fermentation,Charcuterie Expertise,Fermented Meats ,Food Safety Standards,Food Processing Guidelines,Starter Cultures,Chemical Acidification,Water Activity (a_w),Critical Limits,Degree-Hours Method,Foodborne Pathogens,Meat Processing Equipment,Processing Facility Requirements,Hazard Analysis and Critical Control Points (HACCP),Food Preservation Techniques,Temperature Control,Pathogen Reduction,Food Industry Compliance,Documentation Practices,Heat Treatment,pH Control,Food Stability,Consistency in Production,Microbial Testing,Real-time Monitoring,Process Validation,Regulatory Requirements,Verotoxigenic E. coli,Lethality Standards,Product Labelling,Spoilage Prevention,Enterotoxin Detection,Shelf-Stable Products,Moisture to Protein Ratio (MPR)

[mbiometer]

How can I evaluate soil microbial biomass?

Try MICROBIOMETER as your Microbial Biomass Soil Testing Equipment to keep things simple, yet advanced. The journey of understanding your soil's living community is rewarding in itself. To know more details visit https://mbiometer.livepositively.com/how-can-i-evaluate-soil-microbial-biomass/

[labequipment]

Air Sampler

Labmate Air Sampler is a highly reliable air sampler designed with an autoclavable sampling head to avoid any microbial contamination and particle sampling with a volume ranging from 0.01 to 9.0 m³ and sampling Volume of 100 L/min . It allow automatic control the sampling time. Set parameters and view reading on a digital display with real-time monitoring features. It's Data Storage is 256 Sample.

[cryptosexologist]

prev i am now so angry that physics/chemistry isn’t doing better. its like if math had a baby with every bad part of science. sucks doodoo dogshit

[foodagriculturenews]

The food pathogen testing market size is predicted to grow at a CAGR of 8.4% between 2023 and 2028, reaching a value of $22.7 billion by 2028 from a projection of $15.1 billion in 2023.

[the-most-humble-blog]

🧬👻 “You Think You’re You? That’s Adorable.”

You’re not even fully human. You’re a haunted meat golem with Wi-Fi and anxiety.

ACT I — The Lie You’re Living

Ah, yes. You wake up. Brush your teeth. Sip your coffee. Scroll your phone. You feel like a real person with thoughts, memories, preferences.

Cute.

Because here’s the punchline, sweet summer child:

You’re not even 100% human.

ACT II — What You Really Are

You’re a walking, talking orgy of multiple species. Part human. Part bacteria. Part fungus. Part virus. Part ancient ape. And 100% confused spaghetti code pretending to have a soul.

The human body? A biological group project between evolution, gut microbes, parasitic DNA hitchhikers, and ancient mitochondria that used to be their own species.

Your body contains:

More non-human cells than human ones.

Bacteria that outnumber your own cells 10 to 1.

DNA from viruses, ancient fungi, and unclassifiable “dark genome” segments that we literally do not understand.

You are not a person. You’re a biofilm with opinions.

ACT III — You’re a Colony. Not an Individual.

Think about this:

Your thoughts can be influenced by the bacteria in your gut.

Your moods are affected by your microbiome.

Your decisions can shift depending on what fungus you inhaled that day.

Your attraction to people? Might be chemical signals from your skin flora.

You ever get a “gut feeling”?

That might literally be your intestinal bacteria whispering strategy into your brain.

And you thought you were “making a choice.”

ACT IV — Are You Even There?

Let’s go deeper:

You don’t control your heartbeat. You don’t control your dreams. You don’t control what you forget, or when you cry, or what triggers your trauma. You don’t control the timing of your thoughts.

So the question is:

Who the f*ck is actually driving this meat suit?

Because neuroscience doesn’t know. Religion argues. Philosophy hyperventilates. And physics just stares blankly into the void.

ACT V — You Might Be a Ghost. Or Just a Glitch.

You’re either:

A consciousness that’s somehow haunting a nervous system

A chemical puppet with enough complexity to simulate free will

A hallucination of self generated by accidental electro-meat fireworks

Or, worst of all:

A network of sub-selves constantly arguing while pretending they’re one “I.”

Shocking Truth?

Science has no consensus on what consciousness actually is.

Nobody knows if it’s:

An emergent property

A soul

A quantum algorithm

A shared delusion

Or a horrifying accident we’ve decided to romanticize

ACT VI — Logic Tests That Will Wreck You

Ready to lose sleep? Try these reality-breaking diagnostics:

🧠 Logic Trap 1: “When Are You?”

Your brain processes input with a delay. What you’re experiencing right now actually happened a few milliseconds ago. So… if you’re always behind the present… Where is “now”? And who’s watching it?

🧠 Logic Trap 2: “The Ship of Self”

Every 7 years, your cells have completely regenerated. You are literally not made of the same matter you were as a child. If your body changed… and your thoughts changed… What stayed the same? Who’s left?

🧠 Logic Trap 3: “The False First Person”

What if every time you go to sleep, the “you” that wakes up is a copy? You remember yesterday… but so does the copy. Are you just a rebooted save file that thinks it’s original?

🧠 Logic Trap 4: “The Brain In The Room”

The only proof you have that anyone else exists is sensory input. You could be a brain in a jar, hallucinating all this. Can you prove you’re not?

FINAL VERDICT — You’re Not “You.” You’re Just a Temporary Pattern.

A mind is not a soul. It’s a self-updating hallucination stabilized by hormones, trauma, diet, genetics, and luck.

And when you die?

That pattern ends. And everything you called “you” dissolves into meat, memory, and microbial decay.

The ghost leaves. The flesh rots. The world keeps spinning. No refunds. No backups. No explanations.

🔁 Reblog if you’ve ever felt like something else is steering. 👁 Comment if you’ve questioned your reality since age 9. 🧬 Follow if you’re ready to peel back your face and find the universe staring back.

⚖️ LEGAL DISCLAIMER:

This post is intended as philosophical commentary, not psychiatric advice. If you’re spiraling, eat something, touch grass, and don’t take your thoughts too literally. If you feel like nothing is real… congrats. You’re officially more qualified than most philosophers.

[reasonsforhope]

"An international research team has found almost a million potential sources of antibiotics in the natural world.

Research published in the journal Cell by a team including Queensland University of Technology (QUT) computational biologist Associate Professor Luis Pedro Coelho has used machine learning to identify 863,498 promising antimicrobial peptides -- small molecules that can kill or inhibit the growth of infectious microbes.

The findings of the study come with a renewed global focus on combatting antimicrobial resistance (AMR) as humanity contends with the growing number of superbugs resistant to current drugs.

"There is an urgent need for new methods for antibiotic discovery," Professor Coelho, a researcher at the QUT Centre for Microbiome Research, said. The centre studies the structure and function of microbial communities from around the globe.

"It is one of the top public health threats, killing 1.27 million people each year." ...

"Using artificial intelligence to understand and harness the power of the global microbiome will hopefully drive innovative research for better public health outcomes," he said.

The team verified the machine predictions by testing 100 laboratory-made peptides against clinically significant pathogens. They found 79 disrupted bacterial membranes and 63 specifically targeted antibiotic-resistant bacteria such as Staphylococcus aureus and Escherichia coli.

"Moreover, some peptides helped to eliminate infections in mice; two in particular reduced bacteria by up to four orders of magnitude," Professor Coelho said.

In a preclinical model, tested on infected mice, treatment with these peptides produced results similar to the effects of polymyxin B -- a commercially available antibiotic which is used to treat meningitis, pneumonia, sepsis and urinary tract infections.

More than 60,000 metagenomes (a collection of genomes within a specific environment), which together contained the genetic makeup of over one million organisms, were analysed to get these results. They came from sources across the globe including marine and soil environments, and human and animal guts.

The resulting AMPSphere -- a comprehensive database comprising these novel peptides -- has been published as a publicly available, open-access resource for new antibiotic discovery.

[Note: !!! Love it. Open access research databases my beloved.]"

-via Science Daily, June 5, 2024

[covid-safer-hotties]

Performance of N95 Respirators: Filtration Efficiency for Airborne Microbial and Inert Particles - Published June 4, 2010

Yes, you read the date right.

Abstract In 1995 the National Institute for Occupational Safety and Health issued new regulations for nonpowered particulate respirators (42 CFR Part 84). A new filter certification system also was created. Among the new particulate respirators that have entered the market, the N95 respirator is the most commonly used in industrial and health care environments. The filtration efficiencies of unloaded N95 particulate respirators have been compared with those of dust/mist (DM) and dust/fume/mist (DFM) respirators certified under the former regulations (30 CFR Part 11). Through laboratory tests with NaCl certification aerosols and measurements with particle-size spectrometers, N95 respirators were found to have higher filtration efficiencies than DM and DFM respirators and noncertified surgical masks. N95 respirators made by different companies were found to have different filtration efficiencies for the most penetrating particle size (0.1 to 0.3 µm), but all were at least 95% efficient at that size for NaCl particles. Above the most penetrating particle size the filtration efficiency increases with size; it reaches approximately 99.5% or higher at about 0.75 µm. Tests with bacteria of size and shape similar to Mycobacterium tuberculosis also showed filtration efficiencies of 99.5% or higher. Experimental data were used to calculate the aerosol mass concentrations inside the respirator when worn in representative work environments. The penetrated mass fractions, in the absence of face leakage, ranged from 0.02% for large particle distributions to 1.8% for submicrometer-size welding fumes. Thus, N95 respirators provide excellent protection against airborne particles when there is a good face seal.

[mbiometer]

Why On-Site Soil Testing Trumps Soil Microbial Lab Testing Services

While soil microbial lab testing services have helped evaluate soil health, on-site testing has emerged as a better option for a variety of reasons. Adopting on-site testing will surely pave the way for a more productive and ecologically conscious future as we continue to focus on sustainable agriculture and soil health management. For more details visit https://qr.ae/pyVacq

[wayti-blog]

"NASA’s Perseverance rover has found possible hints of ancient life on Mars ― one of the strongest signs yet of Martian life, according to planetary scientists. Dark-rimmed ‘leopard spots’ in a rock studied by the rover last year could be the remains of Martian microbial activity, researchers said at a conference today.

The announcement comes loaded with caveats. Yes, the spots look a lot like those produced by microbes on Earth. But the spots might have formed without the help of living organisms, researchers say, even if they don’t entirely understand the chemical and physical processes on Mars that would have been at work in that case.

For now, the discovery remains a 1 on the scale of 1 to 7 for evaluating claims of extraterrestrial life1: 1 represents the detection of an intriguing signal, and 7 is a slam-dunk confirmation."

"Regardless of how things play out, the discovery is a significant entry in the history of searching for extraterrestrial life — and a test of researchers’ ability to evaluate potential biosignatures on other planets."

continue reading article

[fatehbaz]

"defending civilization against bugs"

lol the mosquito sculpture

see Pratik Chakrabarti's Medicine and Empire: 1600-1960 (2013) and Bacteriology in British India: Laboratory Medicine and the Tropics (2012)

---

Sir Ronald Ross had just returned from an expedition to Sierra Leone. The British doctor had been leading efforts to tackle the malaria that so often killed English colonists in the country, and in December 1899 he gave a lecture to the Liverpool Chamber of Commerce [...]. [H]e argued that "in the coming century, the success of imperialism will depend largely upon success with the microscope."

Text by: Rohan Deb Roy. "Decolonise science - time to end another imperial era." The Conversation. 5 April 2018.

---

---

---

[A]s [...] Diane Nelson explains: The creation of transportation infrastructure such as canals and railroads, the deployment of armies, and the clearing of ground to plant tropical products all had to confront [...] microbial resistance. The French, British, and US raced to find a cure for malaria [...]. One French colonial official complained in 1908: “fever and dysentery are the ‘generals’ that defend hot countries against our incursions and prevent us from replacing the aborigines that we have to make use of.” [...] [T]ropical medicine was assigned the role of a “counterinsurgent field.” [...] [T]he discovery of mosquitoes as malaria and yellow fever carriers reawakened long-cherished plans such as the construction of the Panama Canal (1904-1914) [...]. In 1916, the director of the US Bureau of Entomology and longtime general secretary of the American Association for the Advancement of Science rejoiced at this success as “an object lesson for the sanitarians of the world” - it demonstrated “that it is possible for the white race to live healthfully in the tropics.” [...] The [...] measures to combat dangerous diseases always had the collateral benefit of social pacification. In 1918, [G.V.], president of the Rockefeller Foundation, candidly declared: “For purposes of placating primitive and suspicious peoples, medicine has some decided advantages over machine guns." The construction of the Panama Canal [...] advanced the military expansion of the United States in the Caribbean. The US occupation of the Canal Zone had already brought racist Jim Crow laws [to Panama] [...]. Besides the [...] expansion of vice squads and prophylaxis stations, during the night women were picked up all over the city [by US authorities] and forcibly tested for [...] diseases [...] [and] they were detained in something between a prison and hospital for up to six months [...] [as] women in Panama were becoming objects of surveillance [...].

Text by: Fahim Amir. "Cloudy Swords." e-flux Journal Issue #115. February 2021.

---

---

---

Richard P. Strong [had been] recently appointed director of Harvard’s new Department of Tropical Medicine [...]. In 1914 [the same year of the Panama Canal's completion], just one year after the creation of Harvard’s Department of Tropical Medicine, Strong took on an additional assignment that cemented the ties between his department and American business interests abroad. As newly appointed director of the Laboratories of the Hospitals and of Research Work of United Fruit Company, he set sail in July 1914 to United Fruit plantations in Cuba, Guatemala, Honduras, Costa Rica, and Panama. […] As a shareholder in two British rubber plantations, [...] Strong approached Harvey Firestone, chief executive of the tire and rubber-processing conglomerate that bore his name, in December 1925 with a proposal [...]. Firestone had negotiated tentative agreements in 1925 with the Liberian government for [...] a 99-year concession to optionally lease up to a million acres of Liberian land for rubber plantations. [...]

[I]nfluenced by the recommendations and financial backing of Harvard alumni such as Philippine governor Gen. William Cameron Forbes [the Philippines were under US military occupation] and patrons such as Edward Atkins, who were making their wealth in the banana and sugarcane industries, Harvard hired Strong, then head of the Philippine Bureau of Science’s Biological Laboratory [where he fatally infected unknowing test subject prisoners with bubonic plague], and personal physician to Forbes, to establish the second Department of Tropical Medicine in the United States [...]. Strong and Forbes both left Manila [Philippines] for Boston in 1913. [...] Forbes [US military governor of occupied Philippines] became an overseer to Harvard University and a director of United Fruit Company, the agricultural products marketing conglomerate best known for its extensive holdings of banana plantations throughout Central America. […] In 1912 United Fruit controlled over 300,000 acres of land in the tropics [...] and a ready supply of [...] samples taken from the company’s hospitals and surrounding plantations, Strong boasted that no “tropical school of medicine in the world … had such an asset. [...] It is something of a victory [...]. We could not for a million dollars procure such advantages.” Over the next two decades, he established a research funding model reliant on the medical and biological services the Harvard department could provide US-based multinational firms in enhancing their overseas production and trade in coffee, bananas, rubber, oil, and other tropical commodities [...] as they transformed landscapes across the globe.

Text by: Gregg Mitman. "Forgotten Paths of Empire: Ecology, Disease, and Commerce in the Making of Liberia's Plantation Economy." Environmental History, Volume 22, Number 1. January 2017. [Text within brackets added by me for clarity and context.]

---

---

---

[On] February 20, 1915, [...] [t]o signal the opening of the Panama-Pacific International Exposition (PPIE), [...] [t]he fair did not officially commence [...] until President Wilson [...] pressed a golden key linked to an aerial tower [...] whose radio waves sparked the top of the Tower of Jewels, tripped a galvanometer, [...] swinging open the doors of the Palace of Machinery, where a massive diesel engine started to rotate. [...] [W]ith lavish festivities [...] nineteen million people has passed through the PPIE's turnstiles. [...] As one of the many promotional pamphlets declared, "California marks the limit of the geographical progress of civilization. For unnumbered centuries the course of empire has been steadily to the west." [...] One subject that received an enormous amount of time and space was [...] the areas of race betterment and tropical medicine. Indeed, the fair's official poster, the "Thirteenth Labor of Hercules," [the construction of the Panama Canal] symbolized the intertwined significance of these two concerns [...]. [I]n the 1910s public health and eugenics crusaders alike moved with little or no friction between [...] [calls] for classification of human intelligence, for immigration restriction, for the promotion of the sterilization and segregation of the "unfit," [...]. It was during this [...] moment, [...] that California's burgeoning eugenicist movement coalesced [...]. At meetings convened during the PPIE, a heterogenous group of sanitary experts, [...] medical superintendents, psychologists, [...] and anthropologists established a social network that would influence eugenics on the national level in the years to come. [...]

In his address titled "The Physician as Pioneer," the president-elect of the American Academy of Medicine, Dr. Woods Hutchinson, credited the colonization of the Mississippi Valley to the discovery of quinine [...] and then told his audience that for progress to proceed apace in the current "age of the insect," the stringent sanitary regime imposed and perfected by Gorgas in the Canal Zone was the sine qua non. [...]

Blue also took part in the conference of the American Society for Tropical Medicine, which Gorgas had cofounded five years after the annexation of Cuba, Puerto Rico, and the Philippines. Invoking the narrative of medico-military conquest [...], [t]he scientific skill of the United States was also touted at the Pan-American Medical Congress, where its president, Dr. Charles L. Reed, delivered a lengthy address praising the hemispheric security ensured by the 1823 Monroe Doctrine and "the combined genius of American medical scientists [...]" in quelling tropical diseases, above all yellow fever, in the Canal Zone. [...] [A]s Reed's lecture ultimately disclosed, his understanding of Pan-American medical progress was based [...] on the enlightened effects of "Aryan blood" in American lands. [...] [T]he week after the PPIE ended, Pierce was ordered to Laredo, Texas, to investigate several incidents of typhus fever on the border [...]. Pierce was instrumental in fusing tropical medicine and race betterment [...] guided by more than a decade of experience in [...] sanitation in Panama [...]. [I]n August 1915, Stanford's chancellor, David Starr Jordan [...] and Pierce were the guests of honor at a luncheon hosted by the Race Betterment Foundation. [...] [At the PPIE] [t]he Race Betterment booth [...] exhibit [...] won a bronze medal for "illustrating evidences and causes of race degeneration and methods and agencies of race betterment," [and] made eugenics a daily feature of the PPIE. [...] [T]he American Genetics Association's Eugenics Section convened [...] [and] talks were delivered on the intersection of eugenics and sociology, [...] the need for broadened sterilization laws, and the medical inspection of immigrants [...]. Moreover, the PPIE fostered the cross-fertilization of tropical medicine and race betterment at a critical moment of transition in modern medicine in American society.

Text by: Alexandra Minna Stern. Eugenic Nation: Faults and Frontiers of Better Breeding in Modern America. Second Edition. 2016.

[spacetimewithstuartgary]

Strongest hints yet of biological activity outside the solar system

Astronomers have detected the most promising signs yet of a possible biosignature outside the solar system, although they remain cautious.

Using data from the James Webb Space Telescope (JWST), the astronomers, led by the University of Cambridge, have detected the chemical fingerprints of dimethyl sulfide (DMS) and/or dimethyl disulfide (DMDS), in the atmosphere of the exoplanet K2-18b, which orbits its star in the habitable zone.

On Earth, DMS and DMDS are only produced by life, primarily microbial life such as marine phytoplankton. While an unknown chemical process may be the source of these molecules in K2-18b’s atmosphere, the results are the strongest evidence yet that life may exist on a planet outside our solar system.

The observations have reached the ‘three-sigma’ level of statistical significance – meaning there is a 0.3% probability that they occurred by chance. To reach the accepted classification for scientific discovery, the observations would have to cross the five-sigma threshold, meaning there would be below a 0.00006% probability they occurred by chance.

The researchers say between 16 and 24 hours of follow-up observation time with JWST may help them reach the all-important five-sigma significance. Their results are reported in The Astrophysical Journal Letters.

Earlier observations of K2-18b — which is 8.6 times as massive and 2.6 times as large as Earth, and lies 124 light years away in the constellation of Leo — identified methane and carbon dioxide in its atmosphere. This was the first time that carbon-based molecules were discovered in the atmosphere of an exoplanet in the habitable zone. Those results were consistent with predictions for a ‘Hycean’ planet: a habitable ocean-covered world underneath a hydrogen-rich atmosphere.

However, another, weaker signal hinted at the possibility of something else happening on K2-18b. “We didn’t know for sure whether the signal we saw last time was due to DMS, but just the hint of it was exciting enough for us to have another look with JWST using a different instrument,” said Professor Nikku Madhusudhan from Cambridge’s Institute of Astronomy, who led the research.

To determine the chemical composition of the atmospheres of faraway planets, astronomers analyse the light from its parent star as the planet transits, or passes in front of the star as seen from the Earth. As K2-18b transits, JWST can detect a drop in stellar brightness, and a tiny fraction of starlight passes through the planet’s atmosphere before reaching Earth. The absorption of some of the starlight in the planet’s atmosphere leaves imprints in the stellar spectrum that astronomers can piece together to determine the constituent gases of the exoplanet’s atmosphere.

The earlier, tentative, inference of DMS was made using JWST’s NIRISS (Near-Infrared Imager and Slitless Spectrograph) and NIRSpec (Near-Infrared Spectrograph) instruments, which together cover the near-infrared (0.8-5 micron) range of wavelengths. The new, independent observation used JWST’s MIRI (Mid-Infrared Instrument) in the mid-infrared (6-12 micron) range.

“This is an independent line of evidence, using a different instrument than we did before and a different wavelength range of light, where there is no overlap with the previous observations,” said Madhusudhan. “The signal came through strong and clear.”

“It was an incredible realisation seeing the results emerge and remain consistent throughout the extensive independent analyses and robustness tests,” said co-author Måns Holmberg, a researcher at the Space Telescope Science Institute in Baltimore, USA. 

DMS and DMDS are molecules from the same chemical family, and both are predicted to be biosignatures. Both molecules have overlapping spectral features in the observed wavelength range, although further observations will help differentiate between the two molecules.

However, the concentrations of DMS and DMDS in K2-18b’s atmosphere are very different than on Earth, where they are generally below one part per billion by volume. On K2-18b, they are estimated to be thousands of times stronger - over ten parts per million.

“Earlier theoretical work had predicted that high levels of sulfur-based gases like DMS and DMDS are possible on Hycean worlds,” said Madhusudhan. “And now we’ve observed it, in line with what was predicted. Given everything we know about this planet, a Hycean world with an ocean that is teeming with life is the scenario that best fits the data we have.”

Madhusudhan says that while the results are exciting, it’s vital to obtain more data before claiming that life has been found on another world. He says that while he is cautiously optimistic, there could be previously unknown chemical processes at work on K2-18b that may account for the observations. Working with colleagues, he is hoping to conduct further theoretical and experimental work to determine whether DMS and DMDS can be produced non-biologically at the level currently inferred.

“The inference of these biosignature molecules poses profound questions concerning the processes that might be producing them” said co-author Subhajit Sarkar of Cardiff University.

“Our work is the starting point for all the investigations that are now needed to confirm and understand the implications of these exciting findings,” said co-author Savvas Constantinou, also from Cambridge’s Institute of Astronomy.

“It’s important that we’re deeply sceptical of our own results, because it’s only by testing and testing again that we will be able to reach the point where we’re confident in them,” Madhusudhan said. “That’s how science has to work.”

While he is not yet claiming a definitive discovery, Madhusudhan says that with powerful tools like JWST and future planned telescopes, humanity is taking new steps toward answering that most essential of questions: are we alone?

“Decades from now, we may look back at this point in time and recognise it was when the living universe came within reach,” said Madhusudhan. “This could be the tipping point, where suddenly the fundamental question of whether we’re alone in the universe is one we’re capable of answering.”

TOP IMAGE: Astronomers have detected the most promising signs yet of a possible biosignature outside the solar system, although they remain cautious. Using data from the James Webb Space Telescope (JWST), the astronomers, led by the University of Cambridge, have detected the chemical fingerprints of dimethyl sulfide (DMS) and/or dimethyl disulfide (DMDS), in the atmosphere of the exoplanet K2-18b, which orbits its star in the habitable zone. Credit A. Smith, N. Madhusudhan (University of Cambridge)

LOWER IMAGE: The graph shows the observed transmission spectrum of the habitable zone exoplanet K2-18 b using the JWST MIRI spectrograph. The vertical shows the fraction of star light absorbed in the planet's atmosphere due to molecules in the planet's atmosphere. The data are shown in the yellow circles with the 1-sigma uncertainties. The curves show the model fits to the data, with the black  curve showing the median fit and the cyan curves outlining the 1-sigma intervals of the model fits. The absorption features attributed to dimethyl sulphide and dimethyl disulphide are indicated by the horizontal lines and text. The image behind the graph is an illustration of a hycean planet orbiting a red dwarf star. Credit A. Smith, N. Madhusudhan (University of Cambridge)

[mbari-blog]

Autonomous robots are essential to the future of marine science, engineering, and exploration. 🤖🌊⁠

Understanding the ocean’s complex physical and biological processes requires robust observing systems, like MBARI’s long-range AUV (LRAUV). The LRAUV was designed to fill the need for a mobile underwater robot to observe upper ocean processes. It has been tested over 36,000 hours offshore across a fleet of eight vehicles and has a unique ultra-low energy transit mode, allowing the vehicle to be operated without a support ship.

MBARI engineers have outfitted the LRAUV with various tools, allowing scientists to track and control the platform remotely and collect real-time ocean data through microbial sampling, bioluminescence, active bio-acoustic imaging, water sampling, plankton imaging, and multibeam mapping. ⁠ ⁠ The ocean is critical to life on Earth, but faces a fragile future and a rising tide of threats. Monitoring ocean health is increasingly urgent, but logistically challenging. Scientists need nimble research tools to scale our observations of the ocean and its inhabitants. We envision a future where robotic platforms, like the MBARI LRAUV, can monitor ocean health 24 hours a day, 365 days a year.⁠

Learn more about the LRAUV on our website.

[outofangband]

Possible medicinal plants in Beleriand part one /?

This is a resource I created upon commission for @creativity-of-death regarding medicinal plants that do have more evidence behind them with the treatment of wounds. Thank you again for the commission! Commission information in the bottom of my pinned post

Related post!

I am also very interested in both botany and herblore so I really enjoyed doing this. This post is heavy on plant information and light on world building but I absolutely plan on writing more detailed world building!

This is going to focus primarily on the plant thyme which are primarily used to treat superficial wounds.

Some notes first!

Elves do not suffer full body infection under most circumstances however, wounds can still worsen, become inflamed, and there are factors that can prevent healing which herbs like these can aid in in. I’m going to make a longer post about my thoughts on immunology and elves and the differences between them and humans going through different aspects of human immune systems and the similarities and differences with elven ones but essentially, while I do believe there are microbial infections in the middle earth, elves are largely immune to communicable diseases that affect humans! however, they are not immune to all poisons, venoms and toxins, as we know from certain examples in canon, for example Aredhel’s death

Ethnobotany is notoriously difficult to research as there are so many conflicted sources and it is often difficult to tell what species have been studied, tested, and proved to have medicinal value, which have been proven to not have significant use in this regard and which have simply not been the subject of much research. Especially when it comes to traditional medicinal practices from marginalized cultures and peoples, information is often dismissed, buried or lost. Oral histories or works in translation are often not included in English literature research.

Second note: understanding of the body and of medicine varies tremendously in my opinion throughout the timeline of middle earth. The information included here is largely the information that we know from modern studies, and the language will not necessarily be the same terms of understanding the characters have, for example boards, like “anti-microbial properties” Would largely be understood by first age, elves, and humans to mean plants that assist in the healing of wounds, prevent them from worsening and prevent illness from falling as a result. Throughout history, we see terms like contamination, blood poisoning, and corruption in place of infection before germ theory was widely understood.

OK now for the plants!

Thyme has been proven in some forms to have significant microbial properties. They are some of the best well antimicrobial properties. Thymol a name for oil of thyme* is and has been used in pesticides and medical disinfectants.

Historically, bandages would be coated in oil of thyme to prevent infection, even before the processes of infection were understood. Thyme contains several different subspecies and tends to grow in Mediterranean climates however has been widely naturalized elsewhere.

In Beleriand it likely grew primarily in Ossiriand and Dorthonion but could easy be naturalized in other temperate regions.

* this compound is also found in other plants to varying degrees, but was named after the substance that was first extracted from common thyme

Wormwood, or Artemisia absinthium has also been studied fairly extensively and has been proven to have antimicrobial properties. I know you mentioned spiders and though it has never been tested on spider venoms, it is proven to have anti parasitic properties and has been used to kill other arachnids such as mites and ticks. Interestingly, it has been examined to have neuroprotective properties which might make it useful in antivenoms. These have not been studied as extensively as its antimicrobial properties however.

In Beleriand this is plant that can be easily naturalized throughout temperate regions, especially in fields and foothills. Dimbar, the region of Nargothrond, and the southern hills are some examples.

Calendula (common marigold)

Evidence has shown that topical application can aid in the healing of wounds and in preventing or treating infection. Tinctures and ointments are the most common forms. These flowers grow throughout the temperate world. Overuse could lead to endangerment/extinction easily. Mount Sinai Hospital’s online medical library discusses these properties as does the National Institute of Health. The properties seem to be well proven, especially by ethnobotanical standards!

Marigold primarily grows in warm regions, including warm temperate ones. In Beleriand it probably grew primarily in the west and central regions.

Common tormentil has mild astringent properties which can aid in blood stopping. Creeping jenny or field balm also has similar properties. These both grow throughout temperate climates though tend towards colder regions.

In Beleriand common tormetil could likely grow in Hithlum and in the plains of Eastern Beleriand. Creeping Jenny would likely be found near ponds such as near the Fen of Serech or Twilit Meres.